Development of a database of amino acid sequences for human colon carcinoma proteins separated by two‐dimensional polyacrylamide gel electrophoresis

@article{Ward1990DevelopmentOA,
  title={Development of a database of amino acid sequences for human colon carcinoma proteins separated by two‐dimensional polyacrylamide gel electrophoresis},
  author={Larry D. Ward and J. Hong and Robert H. Whitehead and Richard J. Simpson},
  journal={ELECTROPHORESIS},
  year={1990},
  volume={11}
}
The tandem use of preparative two‐dimensional polyacrylamide gel electrophoresis (2‐DE) and electroblotting onto polyvinylidene difluoride membranes has been employed to rapidly isolate a number of proteins from a crude cell extract of a human colon carcinoma cell line (LIM 1863). The immobilized proteins were located by staining with Coomassie Brilliant Blue R‐250, and selected protein spots were excised and subjected to Edman degradation. Our résults demonstrate that overall sequence yields… Expand
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