Development of a complex scintillation proximity assay for high throughput screening of PPARγ modulators

@article{Wu2005DevelopmentOA,
  title={Development of a complex scintillation proximity assay for high throughput screening of PPARγ modulators},
  author={Bin Wu and Jie Gao and Ming-wei Wang},
  journal={Acta Pharmacologica Sinica},
  year={2005},
  volume={26},
  pages={339-344}
}
AbstractAim:To develop a complex high-throughput screening (HTS) assay based on scintillation proximity assay (SPA) technology for identification of novel peroxisome proliferators-activated receptor gamma (PPARγ) modulators.Methods:Full length PPARγ and retinoid X receptor alpha (RXRα), biotinylated PPAR response element (PPER), [3H]BRL49653 and streptavidin-coated Flash Plate or microbead were used to develop an HTS assay based on SPA technology. This ‘ABCDE’ method was validated against… CONTINUE READING
7 Citations
23 References
Similar Papers

References

Publications referenced by this paper.
Showing 1-10 of 23 references

Expression of peroxisome

  • Y Guan, Y Zhang, L Davis, MD. Breyer
  • outlook. Curr Opin Investig Drugs
  • 2003

tivity for estrogen receptor beta

  • KL Gearing, M Göttlicher, M Teboul, E Widmark, J. Gustafsson
  • J Med Chem
  • 2002

Similar Papers

Loading similar papers…