Development of a chemically defined serum- and protein-free medium for growth of human peripheral lymphocytes.

@article{Shive1986DevelopmentOA,
  title={Development of a chemically defined serum- and protein-free medium for growth of human peripheral lymphocytes.},
  author={William Shive and Frank H Pinkerton and Joan Humphreys and Marcella M. Johnson and William G. Hamilton and Kathleen Shive Matthews},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1986},
  volume={83 1},
  pages={9-13}
}
A chemically defined, protein-free medium (designated CFBI 1000, where CFBI = Clayton Foundation Biochemical Institute) that supports human peripheral lymphocyte proliferation has been developed. This medium allows exploration of individual metabolic differences by varying the medium composition as well as providing a base to explore further the mechanisms of lymphocyte activation in a system initially free of added macromolecular species other than mitogen. The peripheral blood lymphocyte is… CONTINUE READING