Development of a carbon dioxide-capture assay in microtiter plate for aspartyl-beta-hydroxylase.

@article{Zhang1999DevelopmentOA,
  title={Development of a carbon dioxide-capture assay in microtiter plate for aspartyl-beta-hydroxylase.},
  author={J. H. Zhang and Rong Qi and Tian Chen and Thomas D Y Chung and Andrew M Stern and Gregory F. Hollis and Robert A Copeland and Kevin R Oldenburg},
  journal={Analytical biochemistry},
  year={1999},
  volume={271 2},
  pages={137-42}
}
CO2-capture methods have been used for assaying many decarboxylating enzymes including hydroxylation-coupled decarboxylation reactions. The traditional CO2-capture method involves performing the reaction in capped tubes and radiometric measurement of trapped 14CO2 by scintillation counting. In this report, a 14CO2-capture method in a 96-well microtiter plate format has been developed and a phosphor imaging system has been employed for sample measurement. The new assay method has been used… CONTINUE READING