Development of a capillary electrophoresis-based heteroduplex tracking assay to measure in-host genetic diversity of initial and recurrent Plasmodium vivax infections in Cambodia.

@article{Givens2014DevelopmentOA,
  title={Development of a capillary electrophoresis-based heteroduplex tracking assay to measure in-host genetic diversity of initial and recurrent Plasmodium vivax infections in Cambodia.},
  author={Matthew B Givens and Jessica T Lin and Chanthap Lon and Panita Gosi and Meng Chuor Char and Charlotte Anne Lanteri and David L. Saunders and Jonathan J. Juliano},
  journal={Journal of clinical microbiology},
  year={2014},
  volume={52 1},
  pages={298-301}
}
A heteroduplex tracking assay used to genotype Plasmodium vivax merozoite surface protein 1 was adapted to a capillary electrophoresis format, obviating the need for radiolabeled probes and allowing its use in settings where malaria is endemic. This new assay achieved good allelic discrimination and detected high multiplicities of infection in 63 P. vivax… CONTINUE READING