Development of a bone marrow culture for maintenance and growth of normal human B cell precursors.

@article{Wolf1991DevelopmentOA,
  title={Development of a bone marrow culture for maintenance and growth of normal human B cell precursors.},
  author={M L Wolf and James A Buckley and Adam Goldfarb and C L Law and Tucker W Lebien},
  journal={Journal of immunology},
  year={1991},
  volume={147 10},
  pages={3324-30}
}
The absence of long term bone marrow cultures for studying the growth and differentiation of human B cell precursors (BCP) has placed restrictions on the ability to analyze the early stages of human B cell ontogeny. We now describe a bone marrow-derived adherent cell microenvironment that maintains human BCP for several weeks in vitro. The adherent cells are maintained in a serum-free tissue culture medium, and consist of a predominant population of CD10+ fibroblast-like cells and a minor… CONTINUE READING

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