Development of a bioconversion system using Saccharomyces cerevisiae Reductase YOR120W and Bacillus subtilis glucose dehydrogenase for chiral alcohol synthesis.

@article{Yoon2013DevelopmentOA,
  title={Development of a bioconversion system using Saccharomyces cerevisiae Reductase YOR120W and Bacillus subtilis glucose dehydrogenase for chiral alcohol synthesis.},
  author={Shin A. Yoon and Hyung Kwoun Kim},
  journal={Journal of microbiology and biotechnology},
  year={2013},
  volume={23 10},
  pages={
          1395-402
        }
}
Reductases convert some achiral ketone compounds into chiral alcohols, which are important materials for the synthesis of chiral drugs. The Saccharomyces cerevisiae reductase YOR120W converts ethyl-4-chloro-3-oxobutanoate (ECOB) enantioselectively into (R)-ethyl-4-chloro-3- hydroxybutanoate ((R)-ECHB), an intermediate of a pharmaceutical. As YOR120W requires NADPH as a cofactor for the reduction reaction, a cofactor recycling system using Bacillus subtilis glucose dehydrogenase was employed… 
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