Development of a Rapid, Simple Method for Detecting Naegleria fowleri Visually in Water Samples by Loop-Mediated Isothermal Amplification (LAMP)

@inproceedings{Mahittikorn2015DevelopmentOA,
  title={Development of a Rapid, Simple Method for Detecting Naegleria fowleri Visually in Water Samples by Loop-Mediated Isothermal Amplification (LAMP)},
  author={Aongart Mahittikorn and Hirotake Mori and Supaluk Popruk and Amonrattana Roobthaisong and Chantira Sutthikornchai and Khuanchai N Koompapong and Sukhontha Siri and Yaowalark Sukthana and Duangporn Nacapunchai},
  booktitle={PloS one},
  year={2015}
}
Naegleria fowleri is the causative agent of the fatal disease primary amebic meningoencephalitis. Detection of N. fowleri using conventional culture and biochemical-based assays is time-consuming and laborious, while molecular techniques, such as PCR, require laboratory skills and expensive equipment. We developed and evaluated a novel loop-mediated isothermal amplification (LAMP) assay targeting the virulence-related gene for N. fowleri. Time to results is about 90 min and amplification… CONTINUE READING

References

Publications referenced by this paper.
SHOWING 1-10 OF 47 REFERENCES

Rapid identification of Acanthamoeba from contact lens case using loop-mediated isothermal amplification method

U Lek-Uthai, R Passara, K Roongruangchai, P Buddhirakkul, N. Thammapalerd
  • Exp Parasitol
  • 2009
VIEW 7 EXCERPTS
HIGHLY INFLUENTIAL

Pathogenic free-living amoebae: epidemiology and clinical review.

  • Pathologie-biologie
  • 2012
VIEW 3 EXCERPTS
HIGHLY INFLUENTIAL

Survey of Naegleria fowleri, the causative agents of primary amebic meningoencephalitis in stagnant waters around a factory area in Thailand

P Jariya, S Tiewcharoen, V Junnu, P Lertlaituan, V. Suvilhayanvi
  • Siriraj Hosp Gaz
  • 1997
VIEW 3 EXCERPTS
HIGHLY INFLUENTIAL

First Primary Amebic Meningoencephalitis Death Associated with Exposure to TapWater from a U.S. Treated Public DrinkingWater System

J Cope, R Ratard, +3 authors V Hill
  • IDWeek 2014
  • 2014