Development of a Prototype Lateral Flow Immunoassay (LFI) for the Rapid Diagnosis of Melioidosis

@inproceedings{Houghton2014DevelopmentOA,
  title={Development of a Prototype Lateral Flow Immunoassay (LFI) for the Rapid Diagnosis of Melioidosis},
  author={Raymond L. Houghton and Dana E. Reed and Mark Adam Hubbard and Michael J. Dillon and Hongjing Chen and Bart J. Currie and Mark Mayo and Derek S Sarovich and Vanessa Theobald and Direk Limmathurotsakul and Gumphol Wongsuvan and Narisara Chantratita and Sharon J Peacock and Alex R. Hoffmaster and Brea D. Duval and Paul J. Brett and Mary N. Burtnick and David P AuCoin},
  booktitle={PLoS neglected tropical diseases},
  year={2014}
}
Burkholderia pseudomallei is a soil-dwelling bacterium and the causative agent of melioidosis. Isolation of B. pseudomallei from clinical samples is the "gold standard" for the diagnosis of melioidosis; results can take 3-7 days to produce. Alternatively, antibody-based tests have low specificity due to a high percentage of seropositive individuals in endemic areas. There is a clear need to develop a rapid point-of-care antigen detection assay for the diagnosis of melioidosis. Previously, we… CONTINUE READING