Development of a PCR test to diagnose Haemophilus parasuis infections.

  title={Development of a PCR test to diagnose Haemophilus parasuis infections.},
  author={Simone Barros de Oliveira and Lucina Galina and Carlos C Pijoan},
  journal={Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc},
  volume={13 6},
A polymerase chain reaction (PCR) test was developed in order to improve the accuracy and speed of diagnosis of Haemophilus parasuis, an economically important respiratory pathogen that affects swine. The gene sequence of the 16S small subunit ribosomal RNA of H. parasuis (GenBank M75065) was compared with 56 16S sequences of related bacteria, including those frequently isolated from pig tissues. Two species-specific primers were designed: HPS forward and HPS reverse. The predicted size of the… CONTINUE READING
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