Development of a PCR-based tool for detecting immunologically relevant Alt a 1 and Alt a 1 homologue coding sequences.

@article{Gabriel2015DevelopmentOA,
  title={Development of a PCR-based tool for detecting immunologically relevant Alt a 1 and Alt a 1 homologue coding sequences.},
  author={Marta F. Gabriel and Idoia Postigo and Antonio Guti{\'e}rrez-Rodr{\'i}guez and Ester Su{\~n}{\'e}n and C{\^a}ndida Teixeira Tomaz and Jorge Mart{\'i}nez},
  journal={Medical mycology},
  year={2015},
  volume={53 6},
  pages={636-42}
}
Alt a 1 has been recognized as the most important allergen produced by the Pleosporaceae family and is a risk factor for asthma development and/or exacerbation. The aim of this study was to develop a detection tool that is highly specific for species that produced airborne Alt a 1. Based on the highly conserved internal nucleotide region of the several Alt a 1 sequences that are available in GenBank, a pair of primers (Alta1CF/Alta1CR) was designed. A set of primers used by other authors for… CONTINUE READING