Development of a PCR assay for detection of Enterobacteriaceae in foods.

@article{Nakano2003DevelopmentOA,
  title={Development of a PCR assay for detection of Enterobacteriaceae in foods.},
  author={Shigeru Nakano and Toru Kobayashi and Kenichi Funabiki and Atsushi Matsumura and Yasuhiro Nagao and Toshihiro K Yamada},
  journal={Journal of food protection},
  year={2003},
  volume={66 10},
  pages={1798-804}
}
A broad-range PCR assay for the detection of bacteria belonging to the Enterobacteriaceae family was developed in this study. Primers targeting the bacterial 16S rRNA gene were newly designed and used in this PCR assay. To determine the specificity of the assay, 72 different bacterial species (of 49 genera), 2 fungi, 3 animals, and 4 plants were tested. Results were positive for every tested Vibrioaceae or Enterobacteriaceae strain except Proteus mirabilis. For all other bacterial strains and… CONTINUE READING