Development of a Novel PmpD-N ELISA for Chlamydia psittaci Infection.

Abstract

OBJECTIVE Chlamydia psittaci is an avian respiratory pathogen and zoonotic agent. The wide prevalence of C. psittaci poses a threat to the poultry industry and its employees. However, few commercial kits are available for detecting avian antibodies excluding the in-house ELISA kit. In this study, we developed a novel ELISA kit for detecting antibodies against C. psittaci based on the N-terminal fragment of polymorphic outer membrane protein D (PmpD-N) as the coating antigen. METHODS The antigen concentrations, primary antibody, and cut-off value were determined and optimized. The ELISA, designated PmpD-N ELISA, was assessed for sensitivity, specificity, and concordance using sera samples from 48 experimentally infected and 168 uninfected SPF chickens. RESULTS The sensitivity and specificity of PmpD-N ELISA were 97.9%, 100%, respectively, while the concordance was 98.1% as compared to that of MOMP-ELISA. No cross-reaction with positive sera for other avian pathogens was found. Using PmpD-N ELISA, 799/836 clinical samples were positive, including 93.0% and 98.1% positivity in layers and broilers, respectively. CONCLUSION These data indicate that indirect ELISA with PmpD-N as the antigen candidate is a promising approach for the surveillance of C. psittaci infection.

DOI: 10.3967/bes2016.041

Cite this paper

@article{Liu2016DevelopmentOA, title={Development of a Novel PmpD-N ELISA for Chlamydia psittaci Infection.}, author={Shan Liu and Jun Chu and Qiang Zhang and Wei Sun and Tian Yuan Zhang and Cheng He}, journal={Biomedical and environmental sciences : BES}, year={2016}, volume={29 5}, pages={315-22} }