Development of a Multicolor Bioluminescence Imaging Platform to Simultaneously Investigate Transcription Factor NF-κB Signaling and Apoptosis.

Abstract

Here we describe a novel multicolor bioluminescent imaging platform that enables us to simultaneously investigate transcription factor nuclear factor-κB (NF-κB) signalling and apoptosis. We genetically modified the human breast cancer cell line MDA-MB-231 to express green, red, and blue light-emitting luciferases to monitor cell number and viability, NF-κB promoter activity, and to enable specific cell sorting and detection, respectively. Z-DEVD-animoluciferin, the pro-luciferin substrate, was used to determine apoptotic caspase 3/7 activity. We used this multicolored cell line for the in vitro evaluation of natural compounds and in vivo optical imaging of tumor necrosis factor (TNFα)-induced NF-κB activation (Mezzanotte et al., PLoS One 9:e85550, 2014).

DOI: 10.1007/978-1-4939-3813-1_21

Cite this paper

@article{KnolBlankevoort2016DevelopmentOA, title={Development of a Multicolor Bioluminescence Imaging Platform to Simultaneously Investigate Transcription Factor NF-κB Signaling and Apoptosis.}, author={Vicky T. Knol-Blankevoort and Laura Mezzanotte and Martijn J. W. E. Rabelink and Clemens W. G. M. L{\"{o}wik and Eric L. Kaijzel}, journal={Methods in molecular biology}, year={2016}, volume={1461}, pages={255-70} }