Development of a Method to Implement Whole-Genome Bisulfite Sequencing of cfDNA from Cancer Patients and a Mouse Tumor Model

@inproceedings{Maggi2018DevelopmentOA,
  title={Development of a Method to Implement Whole-Genome Bisulfite Sequencing of cfDNA from Cancer Patients and a Mouse Tumor Model},
  author={Elaine C. Maggi and Silvia Gravina and Haiying Cheng and Bilal Piperdi and Ziqiang Yuan and Xiao Dong and Steven K. Libutti and Jan Vijg and Cristina Montagna},
  booktitle={Front. Genet.},
  year={2018}
}
The goal of this study was to develop a method for whole genome cell-free DNA (cfDNA) methylation analysis in humans and mice with the ultimate goal to facilitate the identification of tumor derived DNA methylation changes in the blood. Plasma or serum from patients with pancreatic neuroendocrine tumors or lung cancer, and plasma from a murine model of pancreatic adenocarcinoma was used to develop a protocol for cfDNA isolation, library preparation and whole-genome bisulfite sequencing of ultra… CONTINUE READING
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