Development of a Highly Sensitive Loop-Mediated Isothermal Amplification (LAMP) Method for the Detection of Loa loa

@article{FernndezSoto2014DevelopmentOA,
  title={Development of a Highly Sensitive Loop-Mediated Isothermal Amplification (LAMP) Method for the Detection of Loa loa},
  author={P. Fern{\'a}ndez-Soto and Prosper Obolo Mvoulouga and J. Aku{\'e} and J. L. Ab{\'a}n and Bel{\'e}n Vicente Santiago and M. C. S{\'a}nchez and A. Muro},
  journal={PLoS ONE},
  year={2014},
  volume={9}
}
The filarial parasite Loa loa, the causative agent of loiasis, is endemic in Central and Western Africa infecting 3-13 million people. [...] Key Method Primers for LAMP were designed from a species-specific repetitive DNA sequence from L. loa retrieved from GenBank. Genomic DNA of a L. loa adult worm was used to optimize the LAMP conditions using a thermocycler or a conventional heating block. Amplification of DNA in the LAMP mixture was visually inspected for turbidity as well as addition of fluorescent dye…Expand
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