Development of a Fluorescent Enzyme-Linked DNA Aptamer-Magnetic Bead Sandwich Assay and Portable Fluorometer for Sensitive and Rapid Leishmania Detection in Sandflies

@article{Bruno2013DevelopmentOA,
  title={Development of a Fluorescent Enzyme-Linked DNA Aptamer-Magnetic Bead Sandwich Assay and Portable Fluorometer for Sensitive and Rapid Leishmania Detection in Sandflies},
  author={John Gordon Bruno and Alicia M. Richarte and Taylor Phillips and Alissa A. Savage and Jeffrey C. Sivils and Alex P Greis and Michael W. Mayo},
  journal={Journal of Fluorescence},
  year={2013},
  volume={24},
  pages={267-277}
}
A fluorescent peroxidase-linked DNA aptamer-magnetic bead sandwich assay is described which detects as little as 100 ng of soluble protein extracted from Leishmania major promastigotes with a high molarity chaotropic salt. Lessons learned during development of the assay are described and elucidate the pros and cons of using fluorescent dyes or nanoparticles and quantum dots versus a more consistent peroxidase-linked Amplex Ultra Red (AUR; similar to resazurin) fluorescence version of the assay… CONTINUE READING

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