Development of a DNA vaccine against hirame rhabdovirus and analysis of the expression of immune-related genes after vaccination.

@article{Takano2004DevelopmentOA,
  title={Development of a DNA vaccine against hirame rhabdovirus and analysis of the expression of immune-related genes after vaccination.},
  author={Tomokazu Takano and Akiko Iwahori and Ikuo Hirono and Takashi Aoki},
  journal={Fish & shellfish immunology},
  year={2004},
  volume={17 4},
  pages={367-74}
}
Intramuscular injection of Japanese flounder, Paralichthys olivaceus (average weight approximately 2 g) with 1 and 10 microg of a plasmid DNA vaccine encoding the hirame rhabdovirus (HIRRV) glycoprotein gene (pCMV-HRVg) was found to provide strong protection against HIRRV. We also conducted a real-time PCR analysis to quantify immune-related genes, e.g. MHC… CONTINUE READING