Development of a Chlamydophila psittaci species-specific and genotype-specific real-time PCR

@inproceedings{GEENSa2005DevelopmentOA,
  title={Development of a Chlamydophila psittaci species-specific and genotype-specific real-time PCR},
  author={Tom GEENSa and Angelo DEWITTEa and Nico BOONb and Daisy VANROMPAYa},
  year={2005}
}
  • Tom GEENSa, Angelo DEWITTEa, +1 author Daisy VANROMPAYa
  • Published 2005
A Chlamydophila psittaci species-specific real-time PCR targeting the rDNA ribosomal spacer was developed as well as a genotype-specific real-time PCR targeting the Cp. psittaci outer membrane protein A (ompA) gene. The SYBR Green-based species-specific real-time PCR detected Cp. psittaci genotypes A to F, and the recently discovered E/B genotype. The genotype-specific real-time PCR could easily distinguish genotypes C, D, F by use of TaqMan probes. Genotypes A, B and E could not be… CONTINUE READING
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