Development of a 16S rRNA gene primer and PCR-restriction fragment length polymorphism method for rapid detection of members of the genus Megasphaera and species-level identification.

Abstract

The genus Megasphaera is relevant to the environment, human health and food, and renewable energy for the future. In this study, a primer set was designed for PCR-restriction fragment length polymorphism (RFLP) analyses to detect and identify the members of Megasphaera. Direct detection and identification were achieved for environmental samples and isolates.

DOI: 10.1128/AEM.00359-11

4 Figures and Tables

Cite this paper

@article{Ohnishi2011DevelopmentOA, title={Development of a 16S rRNA gene primer and PCR-restriction fragment length polymorphism method for rapid detection of members of the genus Megasphaera and species-level identification.}, author={Akihiro Ohnishi and Shinko Abe and Shiho Nashirozawa and S. Shimada and Naoshi Fujimoto and Masaharu Suzuki}, journal={Applied and environmental microbiology}, year={2011}, volume={77 15}, pages={5533-5} }