Development of Vitellogenin-ELISA, an In Vivo Bioassay, and Identification of Two Vitellogenesis-Inhibiting Hormones of the Tiger Shrimp Penaeus monodon

@article{Vincent2001DevelopmentOV,
  title={Development of Vitellogenin-ELISA, an In Vivo Bioassay, and Identification of Two Vitellogenesis-Inhibiting Hormones of the Tiger Shrimp Penaeus monodon},
  author={Samuel G. Prakash Vincent and Rainer Keller and Thanumalayaperumal Subramoniam},
  journal={Marine Biotechnology},
  year={2001},
  volume={3},
  pages={561-571}
}
A sandwich enzyme-linked immunosorbent assay (ELISA) for the quantification of vitellogenin in the hemolymph of Penaeus monodon is reported. Lipovitellin from the mature ovary was purified using hydroxylapatite column chromatography and used as the standard protein, which was serially diluted from 3336 to 6.51 μg and gave a linear plot. Sensitivity results showed ELISA was insensitive to samples that did not contain vitellogenin or lipovitellin. Specificity results showed the degree of the… CONTINUE READING
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