Development of Transformation System in Monascus Purpureus using an Autonomous Replication Vector with Aureobasidin A Resistance Gene

@article{Shimizu2005DevelopmentOT,
  title={Development of Transformation System in Monascus Purpureus using an Autonomous Replication Vector with Aureobasidin A Resistance Gene},
  author={Takeo Shimizu and Hiroshi Kinoshita and Takuya Nihira},
  journal={Biotechnology Letters},
  year={2005},
  volume={28},
  pages={115-120}
}
To enhance the variety of genetic tools and thus to promote molecular genetic study, aureobasidin A and its resistance gene were adopted as a new marker system together with the incorporation of the Gateway system to facilitate the introduction of long heterologous DNA fragments into Monascus purpureus. The minimum inhibitory concentration of aureobasidin A against Monascus was 0.05 μg/ml and a transformation efficiency of 17 colonies/μg DNA was obtained by the protoplast-PEG method with the… CONTINUE READING
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SHOWING 1-10 OF 11 REFERENCES

Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold

  • J Sambrook, EF Fritsch, T Maniatis
  • 1989
Highly Influential
2 Excerpts

Strategies for the transformation of filamentous funji

  • B Ruiz-Dı́ez
  • J. Appl. Microbiol
  • 2002
2 Excerpts

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