Development of SNP markers using genotyping-by-sequencing for cultivar identification in rose (Rosa hybrida)

@article{Heo2017DevelopmentOS,
  title={Development of SNP markers using genotyping-by-sequencing for cultivar identification in rose (Rosa hybrida)},
  author={Moon-Sun Heo and Koeun Han and J Kwon and Byoung-Cheorl Kang},
  journal={Horticulture, Environment, and Biotechnology},
  year={2017},
  volume={58},
  pages={292-302}
}
In this study, we developed single nucleotide polymorphism (SNP) markers to identify cultivars of the polyploid Rosa hybrida using genotyping-by-sequencing (GBS). Sequences obtained from GBS libraries of the genomes of 79 rose cultivars were aligned to contigs created by de novo assembly, and these contigs ranged from 200 to 2,591 bp, with an average of 305 bp per contig. We selected 1,778 SNPs from 13,488 putative SNPs. SNP markers that were present in more than 70% of the 79 cultivars were… CONTINUE READING