Development of PCR Method for Determination of Porcine Parvovirus

  title={Development of PCR Method for Determination of Porcine Parvovirus},
  author={Xing Rui-chang},
Objective To develop a PCR method for the determination of porcine parvovirus(PPV).Methods Design a pair of primers specific for PPV,and optimize the condition for PCR by block titration.Amplify gene fragments by PCR using the DNAs extracted from wild and AV7909 strains of PPV as well as other virus strains,such as canine parvovirus(CPV),rat parvovirus(RPV) and minute virus of mice(MVM),as templates respectively.Identify the PCR products by dot hybridization.The developed PCR method was tested… CONTINUE READING