Development of Multicellular Tumor Spheroid (MCTS) Culture from Breast Cancer Cell and a High Throughput Screening Method Using the MTT Assay

@inproceedings{Ho2012DevelopmentOM,
  title={Development of Multicellular Tumor Spheroid (MCTS) Culture from Breast Cancer Cell and a High Throughput Screening Method Using the MTT Assay},
  author={Wan Yong Ho and Swee Keong Yeap and Chai Ling Ho and Raha Abdul Rahim and Noorjahan Banu Mohamed Alitheen},
  booktitle={PloS one},
  year={2012}
}
In comparison to monolayer cells, MCTS has been claimed as more suitable candidate for studying drug penetration due to the high resemblance to solid tumors. However, the cultivation of MCTS is cumbersome, time consuming, and most technique fail to generate spheroids with uniform sizes. Therefore, the application of spheroid cultures in high throughput screening has been rather limiting. Besides, the lack of a well established screening protocol method that is applicable to spheroid could also… CONTINUE READING
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Elephantopus scaber induces cytotoxicity in MCF-7 human breast cancer cells via p53-induced apoptosis

  • WY Ho, SK Yeap, CL Ho, AR Raha, AA Suraini
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