Development of Long Noncoding RNA-Based Strategies to Modulate Tissue Vascularization

Abstract

BACKGROUND Long noncoding ribonucleic acids (lncRNAs) are a subclass of regulatory noncoding ribonucleic acids for which expression and function in human endothelial cells and angiogenic processes is not well studied. OBJECTIVES The authors discovered hypoxia-sensitive human lncRNAs via next-generation ribonucleic acid sequencing and microarray approaches. To address their functional importance in angiogenic processes, several endothelial lncRNAs were characterized for their angiogenic characteristics in vitro and ex vivo. METHODS Ribonucleic acid sequencing and microarray-derived data showed specific endothelial lncRNA expression changes after hypoxia. Validation experiments confirmed strong hypoxia-dependent activation of 2 intergenic lncRNAs: LINC00323 and MIR503HG. RESULTS Silencing of these lncRNA transcripts led to angiogenic defects, including repression of growth factor signaling and/or the key endothelial transcription factor GATA2. Endothelial loss of these hypoxia-driven lncRNAs impaired cell-cycle control and inhibited capillary formation. The potential clinical importance of these endothelial lncRNAs to vascular structural integrity was demonstrated in an ex vivo model of human induced pluripotent stem cell-based engineered heart tissue. CONCLUSIONS The authors report an expression atlas of human hypoxia-sensitive lncRNAs and identified 2 lncRNAs with important functions to sustain endothelial cell biology. LncRNAs hold great promise to serve as important future therapeutic targets of cardiovascular disease.

DOI: 10.1016/j.jacc.2015.07.081

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@inproceedings{Fiedler2015DevelopmentOL, title={Development of Long Noncoding RNA-Based Strategies to Modulate Tissue Vascularization}, author={Jan Fiedler and Kaja Breckwoldt and Christian W. Remmele and Dorothee Hartmann and Marcus T. Dittrich and Angelika Pfanne and Annette Just and Ke Xiao and Meik Kunz and Tobias M{\"{u}ller and Arne Hansen and R. Geffers and Thomas Dandekar and Thomas Eschenhagen and Thomas Thum}, booktitle={Journal of the American College of Cardiology}, year={2015} }