Development of Leishmania donovani stably expressing DsRed for flow cytometry-based drug screening using chalcone thiazolyl-hydrazone as a new antileishmanial target.

@article{Jaiswal2016DevelopmentOL,
  title={Development of Leishmania donovani stably expressing DsRed for flow cytometry-based drug screening using chalcone thiazolyl-hydrazone as a new antileishmanial target.},
  author={A. K. Jaiswal and K. B. Rao and Pragati Kushwaha and Keerti Rawat and Ram K. Modukuri and P. Khare and S. Joshi and Shikha Mishra and A. Rai and K. Sashidhara and A. Dube},
  journal={International journal of antimicrobial agents},
  year={2016},
  volume={48 6},
  pages={
          695-702
        }
}
  • A. K. Jaiswal, K. B. Rao, +8 authors A. Dube
  • Published 2016
  • Biology, Medicine
  • International journal of antimicrobial agents
  • Green fluorescent protein produces significant fluorescence and is extremely stable, however its excitation maximum is close to the ultraviolet range and thus can damage living cells. Hence, Leishmania donovani stably expressing DsRed were developed and their suitability for flow cytometry-based antileishmanial screening was assessed by evaluating the efficacies of standard drugs as well as newly synthesised chalcone thiazolyl-hydrazone compounds. The DsRed gene was successfully integrated at… CONTINUE READING
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