Development of Infrared Imaging to Measure Thermogenesis in Cell Culture: Thermogenic Effects of Uncoupling Protein-2, Troglitazone, and β-Adrenoceptor Agonists

@article{Paulik1998DevelopmentOI,
  title={Development of Infrared Imaging to Measure Thermogenesis in Cell Culture: Thermogenic Effects of Uncoupling Protein-2, Troglitazone, and β-Adrenoceptor Agonists},
  author={Mark Andrew Paulik and Richard G. Buckholz and Mary E. Lancaster and Walter Southwick Dallas and Emily A. Hull-Ryde and James. E. Weiel and James Martin Lenhard},
  journal={Pharmaceutical Research},
  year={1998},
  volume={15},
  pages={944-949}
}
AbstractPurpose. Although the effects of thermogenic agents in cell culture can be measured by direct microcalorimetry, only a few samples can be analyzed over several hours. In this report, we describe a robust non-invasive technique to measure real-time thermogenesis of cells cultured in microtiter plates using infrared thermography. Methods. Yeast were transformed with uncoupling protein-2 (UCP2) or exposed to carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone (FCCP) or rotenone… CONTINUE READING
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