Development of GFP-based biosensors possessing the binding properties of antibodies.

@article{Pavoor2009DevelopmentOG,
  title={Development of GFP-based biosensors possessing the binding properties of antibodies.},
  author={Tej Venkatachalam Pavoor and Yong Ku Cho and Eric V Shusta},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2009},
  volume={106 29},
  pages={11895-900}
}
Proteins that can bind specifically to targets that also have an intrinsic property allowing for easy detection could facilitate a multitude of applications. While the widely used green fluorescent protein (GFP) allows for easy detection, attempts to insert multiple binding loops into GFP to impart affinity for a specific target have been met with limited success because of the structural sensitivity of the GFP chromophore. In this study, directed evolution using a surrogate loop approach and… CONTINUE READING