Development of Fast-Response GCaMP6 Calcium Sensors for Monitoring Neuronal Action Potential

@article{Helassa2016DevelopmentOF,
  title={Development of Fast-Response GCaMP6 Calcium Sensors for Monitoring Neuronal Action Potential},
  author={N. Helassa and Borbala Podor and A. Fine and K. T{\"o}r{\"o}k},
  journal={Biophysical Journal},
  year={2016},
  volume={110}
}
Green Fluorescent-Calmodulin Proteins (GCaMPs) have been the reporters of choice for visualizing neuronal network activity in vivo. With the GCaMP6 generation sufficient brightness was achieved for single action potential (AP) detection1. However, the Ca2+-response kinetics of GCaMP6 probes remained limiting to separating individual APs. The aim was to accelerate the Ca2+ response kinetics of GCaMP6 probes by point mutations in the Ca2+.CaM.RS20 complex2. A series of probes retaining high… Expand
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