Development of ELISAs for irinotecan and its active metabolite SN-38.

  title={Development of ELISAs for irinotecan and its active metabolite SN-38.},
  author={Tetsuya Saita and Hiroshi Fujito and Masato Mori},
  journal={Biological \& pharmaceutical bulletin},
  volume={23 8},
Two highly sensitive and specific enzyme-linked immunosorbent assays (ELISAs) for the determination of 7-ethyl-10-[4-(1-piperidino)-1-piperidino]carbonyloxy-camptothecin (irinotecan) and 7-ethyl-10-hydroxycamptothecin (SN-38), an active metabolite of irinotecan, were developed, which are capable of measuring as low as 16 and 160 pg of each drug/ml, respectively. Anti-irinotecan antibody was obtained by immunizing rabbits with irinotecan conjugated with mercaptosuccinyl bovine serum albumin (MS… 
4 Citations
Determination of topotecan by ELISA.
A highly sensitive ELISA for the determination of (s)-9-dimethylaminomethyl-10-hydroxy-camptothecin (topotecan) capable of measuring as low as 80 pg/ml was developed and suggests that this ELISA can detect the natural amounts of the lactone form.
A specific and sensitive assay for gefitinib using the enzyme-linked immunosorbent assay in human serum.
The specificity and sensitivity of the ELISA for gefitinib should provide a valuable new tool for use in pharmacokinetic and toxicity studies of gefITinib.
A fast method for the detection of irinotecan in plasma samples by combining solid phase extraction and differential pulse voltammetry
The suitability of the method proposed here for clinical applications was verified by determining the concentration of CPT-11 in plasma samples of an oncological patient, collected after 30 and 180 min from the infusion of the drug.
Antitumor drugs possessing topoisomerase I inhibition: applicable separation methods.
  • T. Oguma
  • Biology, Chemistry
    Journal of chromatography. B, Biomedical sciences and applications
  • 2001