Development of Bimolecular Fluorescence Complementation Using Dronpa for Visualization of Protein–Protein Interactions in Cells

@article{Lee2010DevelopmentOB,
  title={Development of Bimolecular Fluorescence Complementation Using Dronpa for Visualization of Protein–Protein Interactions in Cells},
  author={You Ri Lee and Jong-Hwa Park and Soo-Hyun Hahm and Lin-Woo Kang and Ji Hyung Chung and Ki-Hyun Nam and Kwang Yeon Hwang and Ick Chan Kwon and Ye Sun Han},
  journal={Molecular Imaging and Biology},
  year={2010},
  volume={12},
  pages={468-478}
}
We developed a bimolecular fluorescence complementation (BiFC) strategy using Dronpa, a new fluorescent protein with reversible photoswitching activity and fast responsibility to light, to monitor protein–protein interactions in cells. Dronpa was split at residue Glu164 in order to generate two Dronpa fragments [Dronpa N-terminal: DN (Met1–Glu164), Dronpa C-terminal: DC (Gly165–Lys224)]. DN or DC was separately fused with C terminus of hHus1 or N terminus of hRad1. Flexible linker [(GGGGS)×2… CONTINUE READING
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