Development of 5' nuclease real-time PCR assays for the rapid identification of the burkholderia mallei//burkholderia pseudomallei complex.

@article{Tomaso2004DevelopmentO5,
  title={Development of 5' nuclease real-time PCR assays for the rapid identification of the burkholderia mallei//burkholderia pseudomallei complex.},
  author={Herbert Tomaso and Holger Christian Scholz and Sascha Al Dahouk and Tyrone Leslie Pitt and Thomas M Treu and Heinrich Neubauer},
  journal={Diagnostic molecular pathology : the American journal of surgical pathology, part B},
  year={2004},
  volume={13 4},
  pages={247-53}
}
Burkholderia pseudomallei is the causative agent of melioidosis and was classified as a biologic agent by the Centers for Disease Control and Prevention (Atlanta, GA). Acute melioidosis has a case fatality rate of >40%, and septicemia is fatal in up to 90%. The aim of the study was to design 5'-nuclease real-time PCR assays for the rapid and reliable identification of the B. mallei/B. pseudomallei complex. Real-time PCR assays using TaqMan probes targeting the 16S rDNA and fliC were developed… CONTINUE READING

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