Development of 3T3‐like lines from Balb/c mouse embryo cultures: Transformation susceptibility to SV40

@article{Aaronson1968DevelopmentO3,
  title={Development of 3T3‐like lines from Balb/c mouse embryo cultures: Transformation susceptibility to SV40},
  author={Stuart A Aaronson and George J. Todaro},
  journal={Journal of Cellular Physiology},
  year={1968},
  volume={72}
}
Balb/c mouse embryo cultures that are carried under a rigid transfer schedule that minimizes cell‐cell contact evolve into permanent lines that possess properties very similar to 3T3. From the same embryo cultures, a transfer schedule where cell‐cell contact is extensive leads to lines (Balb/3T12) that form multiple cell layers and have saturation densities up to 25‐fold higher than the Balb/3T3 lines. All the lines are hypotetraploid. 
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  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1968
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