Development of 16S rRNA-gene-targeted group-specific primers for the detection and identification of predominant bacteria in human feces.

@article{Matsuki2002DevelopmentO1,
  title={Development of 16S rRNA-gene-targeted group-specific primers for the detection and identification of predominant bacteria in human feces.},
  author={Takahiro Matsuki and Koichi Watanabe and Junji Fujimoto and Yukiko Miyamoto and Toshihiko Takada and Kazumasa Matsumoto and Hiroshi Oyaizu and Ryuichiro Tanaka},
  journal={Applied and environmental microbiology},
  year={2002},
  volume={68 11},
  pages={5445-51}
}
For the detection and identification of predominant bacteria in human feces, 16S rRNA-gene-targeted group-specific primers for the Bacteroides fragilis group, Bifidobacterium, the Clostridium coccoides group, and Prevotella were designed and evaluated. The specificity of these primers was confirmed by using DNA extracted from 90 species that are commonly found in the human intestinal microflora. The group-specific primers were then used for identification of 300 isolates from feces of six… CONTINUE READING
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