Development of 15 SSR polymorphic markers for the endangered Ameroglossum pernambucense Eb. Fischer, S. Vogel & A. V. Lopes (Scrophulariaceae), and cross-transferability in congeneric taxa

  title={Development of 15 SSR polymorphic markers for the endangered Ameroglossum pernambucense Eb. Fischer, S. Vogel \& A. V. Lopes (Scrophulariaceae), and cross-transferability in congeneric taxa},
  author={Artur M. Wanderley and Santelmo Vasconcelos and Bruno Huettel and Isabel Cristina Machado and Ana Maria Benko-Iseppon},
  journal={Brazilian Journal of Botany},
Ameroglossum (Scrophulariaceae) is an endemic genus from northeastern Brazil patchily distributed in granitic rock outcrops (inselbergs) with two species described: the endangered A. pernambucense Eb. Fischer, S. Vogel & A. V. Lopes and the narrow endemic A. manoel-felixii L. P. Felix & E. M. Almeida. We developed 15 polymorphic nuclear microsatellite markers for A. pernambucense using pyrosequencing and successfully cross-amplified 14 of these loci in A. manoel-felixii and a taxonomically… 
Genome survey and development of 18 microsatellite markers to assess genetic diversity in Spondias tuberosa Arruda Câmara (Anacardiaceae) and cross-amplification in congeneric species
It is concluded that the microsatellite markers developed in this study are useful in genetic population and conservation studies, as well as for investigating the hybrid origins of Spondias species.
The roles of geography and environment in divergence within and between two closely related plant species inhabiting an island‐like habitat
This work tested the relative contribution of geographic and environmental isolation in genetic and phenotypic divergence within and between two species of the figwort Ameroglossum inhabiting spatially isolated habitats, known as inselbergs.


Reproductive ecology of Ameroglossum pernambucense (Scrophulariaceae): is this ornithophilous and threatened shrub highly adapted to a naturally fragmented habitat?
Study of two populations of A. pernambucense determined if three reproductive features facilitate the colonisation of new areas and the gene flow among the outcrops, and interpreted the reproductive traits observed as highly adapted to its fragmented condition.
Population differentiation and species cohesion in two closely related plants adapted to neotropical high‐altitude ‘inselbergs’, Alcantarea imperialis and Alcantarea geniculata (Bromeliaceae)
The results indicate a high potential for inselbergs as venues for studies of the molecular ecology and genetics of continental radiations, such as the one that gave rise to the extraordinary diversity of adaptive strategies and phenotypes seen in Bromeliaceae.
Rock outcrop orchids reveal the genetic connectivity and diversity of inselbergs of northeastern Brazil
Both orchids Epidendrum cinnabarinum and E. secundum showed similar patterns of genetic diversity and structure, highlighting the importance of seed-restricted gene flow and genetic drift as drivers of plant diversification in terrestrial islands such as inselbergs.
Genetic structure of the xerophilous bromeliad Pitcairnia geyskesii on inselbergs in French Guiana – a test of the forest refuge hypothesis
Inselbergs are isolated granitic rock outcrops that provide distinctive ecological conditions. In northern South America they rise above the surrounding rainforest. Among inselberg specialists,
Ameroglossum, a new monotypic genus of Scrophulariaceae-Scrophularioideae from Brazil
The new monotypic genus Ameroglossum EB. FISCHER, S. VOGEL & A. LOPES with the new species A. pernambucense EB.FISCHER, S. VOGEL & A. LOPES from Brazil is described. It differs from the related
A New Species of Ameroglossum (Scrophulariaceae) Growing on Inselbergs in Northeastern Brazil
Ameroglossum manoel-felixii, a new species of AmeroglossUM, is described and illustrated here, and morphologically compared with A. pernambucense.
Conserving the evolutionary potential of California valley oak (Quercus lobata Née): a multivariate genetic approach to conservation planning
These findings, based on the distribution of multivariate genotypes and allelic richness, identify areas with distinctive histories and genetic composition that should be given priority in reserve network design, especially because these areas also overlap with landscape change and little degree of protection.
Extensive long-distance pollen dispersal and highly outcrossed mating in historically small and disjunct populations of Acacia woodmaniorum (Fabaceae), a rare banded iron formation endemic
It is proposed that maintenance of genetic connectivity through habitat and pollinator conservation will be a key factor in the persistence of this and other historically rare species with similar extensive long-distance pollen dispersal and highly outcrossed mating systems.
DNA fingerprinting in plants: principles, methods, and applications
The PCR Generation: Molecular Markers Based on In Vitro DNA Amplification Principle of the PCR Cleaved Amplified Polymorphic Sequences and PCR and Hybridization: Combinatory Techniques.
Arlequin (version 3.0): An integrated software package for population genetics data analysis
A completely new graphical interface written in C++, a more robust semantic analysis of input files, and two new methods: a Bayesian estimation of gametic phase from multi-locus genotypes, and an estimation of the parameters of an instantaneous spatial expansion from DNA sequence polymorphism are introduced.