Development of 1-cell embryos from different strains of mice in CZB medium.

@article{Chatot1990DevelopmentO1,
  title={Development of 1-cell embryos from different strains of mice in CZB medium.},
  author={Clare L Chatot and Johnnye L Lewis and Ismael Torres and Carol Ann Ziomek},
  journal={Biology of reproduction},
  year={1990},
  volume={42 3},
  pages={432-40}
}
One-cell embryos from several different strains of mice have been cultured to the blastocyst stage in CZB medium. CZB medium can be used to culture CF1 x B6SJLF1/J 1-cell embryos to the blastocyst stage provided glucose is introduced into the medium on Day 3 of culture. The amount of glucose required for embryo development was titrated using a concentration range of 5.5 to 49.5 mM. With the exception of the highest concentration, all glucose levels tested supported 65-85% development to the… CONTINUE READING
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