Development changes occurring in the lipids of ram epididymal spermatozoa plasma membrane.

@article{Parks1985DevelopmentCO,
  title={Development changes occurring in the lipids of ram epididymal spermatozoa plasma membrane.},
  author={John E. Parks and Roy H. Hammerstedt},
  journal={Biology of reproduction},
  year={1985},
  volume={32 3},
  pages={
          653-68
        }
}
Ram spermatozoa were obtained from different regions (caput, corpus, and cauda) of the epididymis and their plasma membrane was removed using a nitrogen cavitation treatment (750 psi, 10 min equilibration at 4 degrees C). Membrane was recovered after sucrose gradient centrifugation and identified using 125I-succinylated concanavalin A (125I-succConA) as a surface marker. Based on fluorescein isothiocyanate-succConA (FITC-succConA) labeling and electron microscopy, cavitation removed plasma… 
Isolation and characterization of the plasma membrane of rat cauda epididymal spermatozoa.
TLDR
Differences in the lipid composition of rat vesicles described here and similar vesicle preparations isolated from ram and boar spermatozoa (described previously) are discussed.
Vesicular transfer of membrane components to bovine epididymal spermatozoa
TLDR
The results substantiate the presumption that PMCA4 is transferred to spermatozoa via epididymosomes and indicate significant changes in the lipid and protein composition of epididcyal sperm following interaction with epididmosomes.
Correlation between changes in rat sperm membrane lipids, protein, and the membrane physical state during epididymal maturation.
TLDR
The data suggest that the apparent fluidity and biochemical composition of the sperm membrane change during epididymal maturation.
Lipid composition of the membrane released after an in vitro acrosome reaction of epididymal boar sperm
TLDR
The membrane fraction that was released from caudal boar sperm undergoing an in vitro acrosome-like reaction was isolated and characterized with respect to density, marker enzymes and lipid composition, which will allow a better understanding of the mechanism of the sperm acrosomes reaction.
Lipid diffusion in the plasma membrane of ram and boar spermatozoa during maturation in the epididymis measured by fluorescence recovery after photobleaching
TLDR
The technique of fluorescence recovery after photobleaching (FRAP) is used to measure diffusion of the lipid reporter probe ODAF in spermatozoa from two species: ram and boar, where substantial changes in membrane lipids occur during passage through the epididymis, and there are relatively few changes.
Distribution of filipin-sterol complexes in the plasma membrane of stallion spermatozoa during the epididymal maturation process.
TLDR
The presence and distribution of cholesterol in mature and immature epididymal spermatozoa was analyzed using filipin as a cytochemical tool in freeze-fracture replicas and thin section preparations, revealing a specific organization in a functionally specialized area of the gamete.
Phospholipid composition of isolated guinea pig sperm outer acrosomal membrane and plasma membrane during capacitation in vitro.
TLDR
Data provide evidence that changes in the plasma membrane phospholipid composition may be associated with guinea pig sperm capacitation.
Fatty acid content in epididymal fluid and spermatozoa during sperm maturation in dogs
TLDR
These findings demonstrate the influence of the fatty acid profile during sperm maturation, assigning physical and chemical changes in sperm cells, essential for fertilization.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 75 REFERENCES
Modification of ram sperm membranes during epididymal transit.
Our previous research established that membrane physical properties of testicular and cauda epididymal sperm differ in many respects. The data presented herein establish the region of the epididymis
Lipid composition and metabolism in testicular and ejaculated ram spermatozoa.
TLDR
It is suggested that lipids are a source of substrate for spermatozoa during their passage through the epididymis as well as radioactivity from glucose into formate than into acetate, whereas a higher proportion of radioactivity was found in acetate in ejaculated cells.
Surface changes in rat spermatozoa during epididymal transit.
TLDR
Several different approaches were employed to evaluate differences in the plasma membrane of spermatozoa from the caput and cauda epididymis of the rat, and surface differences were established by comparison of externally oriented surface proteins accessible to lactoperoxidase-catalyzed iodination.
Purification and partial characterization of plasma membranes from bovine spermatozoa.
TLDR
It is indicated that nitrogen cavitation of bovine epididymal spermatozoa can result in significant contamination of plasma membrane fractions by cytoplasmic droplet membranes unless appropriate differential centrifugation is used to separate the membrane fractions.
Post-testicular developmental changes in the ram sperm cell surface and their relationship to luminal fluid proteins of the reproductive tract.
TLDR
Results provide direct biochemical evidence that spermatozoa undergo extensive surface modifications during passage through the epididymis where they become almost totally dependent on exogenous substrates for survival and develop the capacity for coordinated movement.
Lipid changes in boar spermatozoa during epididymal maturation with some observations on the flow and composition of boar rete testis fluid.
TLDR
The flow rate and composition of boar rete testis fluid were similar to those in other species studied previously, but the level of phospholipid phosphorus was slightly higher in ejaculated spermatozoa than in epididymal spermatozosa.
...
1
2
3
4
5
...