Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro.

Abstract

In this article we present validation of a real-time RT-PCR method to quantitate mRNA expression levels of atrial natriuretic peptide and c-fos in an in vitro model of cardiac hypertrophy. This method requires minimal sample and no postreaction manipulation. In real-time RT-PCR a dual-labeled fluorescent probe is degraded concomitant with PCR amplification… (More)

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Cite this paper

@article{Winer1999DevelopmentAV, title={Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro.}, author={Jane Winer and Choong Ki Jung and I Shackel and Paul Williams}, journal={Analytical biochemistry}, year={1999}, volume={270 1}, pages={41-9} }