Development and validation of real-time one-step reverse transcription-PCR for the detection and typing of dengue viruses.

Abstract

BACKGROUND Dengue virus, transmitted by mosquitoes, causes every year 50 million cases of dengue fever. A standardize method for early diagnosis is still needed for clinical diagnosis and epidemiological studies. OBJECTIVE To develop and validate for sensitivity, specificity, linearity and precision real-time one-step RT-PCR for the detection of dengue… (More)
DOI: 10.1016/j.jcv.2009.02.010

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