Development and validation of an ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the quantitative determination of rhamnolipid congeners

@article{Rudden2015DevelopmentAV,
  title={Development and validation of an ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the quantitative determination of rhamnolipid congeners},
  author={Michelle Rudden and Konstantina Tsauosi and Roger Marchant and Ibrahim M Banat and Thomas J. Smyth},
  journal={Applied Microbiology and Biotechnology},
  year={2015},
  volume={99},
  pages={9177-9187}
}
Rhamnolipids (RLs) are synthesised as a complex mixture of congeners comprising either one or two molecules of rhamnose glycosidically linked to a dimer of 3-hydroxy fatty acids varying in chain length and degree of saturation. Currently, HPLC-MS/MS is the most precise and accurate method for RL determination, while accurate quantification is limited. In this study, a rapid ultra pressure liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the… CONTINUE READING
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