Development and validation of an UPLC-MS/MS method for the quantification of columbin in biological matrices: Applications to absorption, metabolism, and pharmacokinetic studies.

@article{Yang2015DevelopmentAV,
  title={Development and validation of an UPLC-MS/MS method for the quantification of columbin in biological matrices: Applications to absorption, metabolism, and pharmacokinetic studies.},
  author={Guangyi Yang and Song Gao and Rongjin Sun and Taijun Yin and Ming Hu},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2015},
  volume={1002},
  pages={13-8}
}
The aim of this study is to develop a sensitive UPLC-MS/MS method to quantify columbin in biological sample. Chromatographic separation was accomplished using Waters UPLC BEH C18 column with acetonitrile and 0.1% of formic acid in water as the mobile phases. The mass analysis was performed on an API 5500 Qtrap mass spectrometer via multiple reaction… CONTINUE READING