Development and validation of a triplex real-time PCR for rapid detection and specific identification of M. avium sub sp. paratuberculosis in faecal samples.

@article{Irenge2009DevelopmentAV,
  title={Development and validation of a triplex real-time PCR for rapid detection and specific identification of M. avium sub sp. paratuberculosis in faecal samples.},
  author={Leonid Irenge and Karl Walravens and Marc Govaerts and Jacques Godfroid and Val{\'e}rie Rosseels and Kris Huygen and Jean-Luc Gala},
  journal={Veterinary microbiology},
  year={2009},
  volume={136 1-2},
  pages={166-72}
}
A triplex real-time (TRT-PCR) assay was developed to ensure a rapid and reliable detection of Mycobacterium avium subsp. paratuberculosis (Map) in faecal samples and to allow routine detection of Map in farmed livestock and wildlife species. The TRT-PCR assay was designed using IS900, ISMAP02 and f57 molecular targets. Specificity of TRT-PCR was first confirmed on a panel of control mycobacterial Map and non-Map strains and on faecal samples from Map-negative cows (n=35) and from Map-positive… CONTINUE READING

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Development and validation of a triplex real-time PCR for rapid detection and specific identification of M. avium sub sp. paratuberculosis in faecal samples

  • Irenge, L.M
  • 2008

Veterinary Microbiology xxx

  • L. M. Irenge
  • 2008

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