Development and validation of a specific radioimmunoassay for PHI in plasma.

  title={Development and validation of a specific radioimmunoassay for PHI in plasma.},
  author={Jan Fahrenkrug and Jesper Holst Pedersen},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  volume={143 3},
A sensitive and specific radioimmunoassay for the recently isolated neuropeptide with N-terminal histidine and C-terminal isoleucine amide (PHI) has been developed which can detect 3.0 pmol/l of the peptide in plasma. The routine antiserum produced in rabbit had a titre of 1:800 000 and recognized the sequence 3-8 of the 27 amino acid peptide. Labelling of PHI with 125I was performed by the chloramine T method, and labelled PHI was separated from unlabelled on an octadecylsilyl (ODC)-silica… CONTINUE READING

From This Paper

Figures, tables, results, connections, and topics extracted from this paper.
13 Extracted Citations
0 Extracted References
Similar Papers

Citing Papers

Publications influenced by this paper.
Showing 1-10 of 13 extracted citations

Similar Papers

Loading similar papers…