Development and validation of a simple, fast, isocratic stability indicating RP-HPLC-UV method for the determination of chlorhexidine and its impurity para-chloroaniline in bulk and finished product

@article{Mohammed2017DevelopmentAV,
  title={Development and validation of a simple, fast, isocratic stability indicating RP-HPLC-UV method for the determination of chlorhexidine and its impurity para-chloroaniline in bulk and finished product},
  author={TarigG. Mohammed and Mubashira Aziz},
  journal={IOSR Journal of Pharmacy},
  year={2017},
  volume={07},
  pages={01-08}
}
A simple, isocraticRP HPLC-UV method was developed for the simultaneous determination of chlorhexidine (CHD) and p-Chloroaniline (pCA) inchlorhexidine mouth rinses. An excellent separation obtainedbyC18 column (200mm × 4.6 mm, 3μm). Mobile phasewas acetate buffer:methanol in a 45:55 ratio,flowrate was 1.0 ml/min. Both ingredient and an impuritywere detected at 254 nm,injection volume was 20μl and the analysis temperature was room temperature.Resolution4.7,retention times was3.1min and 5.7 min… 
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We described a simple and rapid method to quantify simultaneously chlorhexidine (CHD) and its major metabolite, para Chloroaniline (pCA) by HPLC with UV detection without the additional need of
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TLDR
This paper reports a new RP-HPLC method using phenacetin internal standard (IS) for simultaneous determination of chlorhexidine (CH) and its impurity 4-chloroaniline (4-CA), which has been used for investigating the stability of CH preparation.
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TLDR
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TLDR
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