Development and validation of a quantitative real time PCR assay for BK virus.

@article{Mitui2013DevelopmentAV,
  title={Development and validation of a quantitative real time PCR assay for BK virus.},
  author={Midori Mitui and Nora Kristine Leos and Damon Lacey and Christopher D Doern and Beverly Barton Rogers and Jason J. Y. Park},
  journal={Molecular and cellular probes},
  year={2013},
  volume={27 5-6},
  pages={230-6}
}
Several studies have shown that BK viral load in plasma and urine are reliable markers for the detection of BK virus associated nephropathy (BKVAN) in renal transplant patients. We developed a quantitative real time PCR assay based on TaqMan technology for the measurement of BK viral load in plasma and urine. Considering the high similarity of the nucleotide sequence of the BK virus (BKV) with the JC virus (JCV), we designed this assay to specifically amplify BKV. We determined the viral DNA… CONTINUE READING