Development and validation of a quantitative real-time PCR assay using fluorescence resonance energy transfer technology for detection of Aspergillus fumigatus in experimental invasive pulmonary aspergillosis.

@article{OSullivan2003DevelopmentAV,
  title={Development and validation of a quantitative real-time PCR assay using fluorescence resonance energy transfer technology for detection of Aspergillus fumigatus in experimental invasive pulmonary aspergillosis.},
  author={Cathal E O'Sullivan and Miki Kasai and Andrea Francesconi and Vidmantas Petraitis and Ruta Petraitiene and Amy M. Kelaher and Alia A Sarafandi and Thomas J Walsh},
  journal={Journal of clinical microbiology},
  year={2003},
  volume={41 12},
  pages={
          5676-82
        }
}
Invasive pulmonary aspergillosis (IPA) is a frequently fatal infection in immunocompromised patients that is difficult to diagnose. Present methods for detection of Aspergillus spp. in bronchoalveolar lavage (BAL) fluid and in tissue vary in sensitivity and specificity. We therefore developed an A. fumigatus-specific quantitative real-time PCR-based assay utilizing fluorescent resonance energy transfer (FRET) technology. We compared the assay to quantitative culture of BAL fluid and lung tissue… CONTINUE READING
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