Development and validation of a gas chromatography-flame ionization detection method for quantifying sucrose in equine serum.

@article{Hewetson2014DevelopmentAV,
  title={Development and validation of a gas chromatography-flame ionization detection method for quantifying sucrose in equine serum.},
  author={Michael Hewetson and Kaisa Aaltonen and R-M. Tulamo and S D Priya Sankari},
  journal={Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc},
  year={2014},
  volume={26 2},
  pages={
          232-9
        }
}
A simple and accurate method for quantifying sucrose in equine serum that can be applied to sucrose permeability testing in the horse was developed and validated using gas chromatography with flame ionization detection. The assay provided an acceptable degree of linearity, accuracy, and precision at concentrations of sucrose as low as 2.34 μmol/l and as high as 20.45 μmol/l. Percentage recovery of sucrose from serum ranged from 89% to 102%; repeatability and intermediate precision (relative… CONTINUE READING
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