Development and validation of a fluorescence technology for both primary and secondary screening of kinases that facilitates compound selectivity and site-specific inhibitor determination.

@article{Morgan2004DevelopmentAV,
  title={Development and validation of a fluorescence technology for both primary and secondary screening of kinases that facilitates compound selectivity and site-specific inhibitor determination.},
  author={A. Morgan and T. J. Mccauley and Michael L. Stanaitis and M. Mathrubutham and S. Millis},
  journal={Assay and drug development technologies},
  year={2004},
  volume={2 2},
  pages={
          171-81
        }
}
The IQ Technology has been developed to serve as a homogeneous, universal detection platform for HTS of kinases and phosphatases. The technology is a direct, noncompetitive assay format that does not require antibodies or radioactive reagents to measure phosphorylation state. Fluorophore-labeled peptides are used as enzyme substrates, and kinase or phosphatase activity is quantitated by direct measurement of the phosphorylation state of the substrate. Phosphorylation is measured by the change… Expand
A Fluorescence Lifetime–Based Binding Assay to Characterize Kinase Inhibitors
Three Mechanistically Distinct Kinase Assays Compared: Measurement of Intrinsic ATPase Activity Identified the Most Comprehensive Set of ITK Inhibitors
Real-time fluorogenic kinase assay using protein as substrate.
Use of pseudosubstrate affinity to measure active protein kinase A.
...
1
2
3
...

References

SHOWING 1-10 OF 28 REFERENCES
Inhibitors of protein tyrosine phosphorylation: preliminary assessment of activity by time-resolved fluorescence.
...
1
2
3
...