Development and validation of a fast and sensitive UPLC-MS/MS method for the quantification of six probe metabolites for the in vitro determination of cytochrome P450 activity.

Abstract

A fast and sensitive UPLC-MS/MS method was developed and validated for the simultaneous quantification of six probe metabolites for the in vitro cytochrome P450 activity determination in hepatic microsomes from patients with hepatic impairment. The metabolites acetaminophen (CYP1A2), 4'-hydroxy-mephenytoin (CYP2C19), 4-hydroxy-tolbutamide (CYP2C9… (More)
DOI: 10.1016/j.talanta.2011.11.083

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Cite this paper

@article{Bock2012DevelopmentAV, title={Development and validation of a fast and sensitive UPLC-MS/MS method for the quantification of six probe metabolites for the in vitro determination of cytochrome P450 activity.}, author={Lies De Bock and Koen Boussery and Pieter J Colin and Julie de Smet and Huybrecht T'jollyn and Jan F P Van Bocxlaer}, journal={Talanta}, year={2012}, volume={89}, pages={209-16} }